Overwinter transplant production for extended season organic cropping (OF 0144)

The objectives of this project (Defra project OF0144), lead by EFRC, were to: • identify acceptable organic fungicide products to control mildew in transplant production • produce integrated organic transplant production systems for brassica, allium and lettuce over the autumn and winter period by identifying optimum cell/block sizes in relation to nutrient requirements and sources, growing media formulation, and supplementary feeding and watering • evaluate developed transplant production protocols during the winter period • undertake technology transfer and dissemination of the results Results: Protocols were tested for a range of crop species and varieties, growing media, cell size and feeding regimes over the three seasons under experimental and commercial conditions. It is possible to produce transplants of a suitable quality over the winter period although propagation time is generally longer than at more favourable times of year. With respect to identifying acceptable fungicide products • a range of fungicidal products was identified including L-Carvone, Mycosin, fennel and clove oils that showed potential in controlling mildew on a range of crop species. However the work also raised the question of the suitability of the use of such materials in organic systems which has not been resolved. • work on spectral filters found no benefits to their use in transplant production • cell size has minimal effect on disease spread As far as integrated organic transplant production systems are concerned: • production time was longer overwinter than in spring • lettuce was relatively easy to produce in a range of media and block sizes with no supplementary feed being necessary • similarly cabbage was relatively easy to produce although feeding was required • cauliflower was produced using smaller cell size and full nutrient compos

Analysis and Definition of the close-to-crop Area in Relation to Robotic Weeding

The objective of this paper is to analyse and define the field conditions close to the crop plants of sugar beet (Beta vulgaris L.). The aim is to use this study for the choice and development of new physical weeding methods to target weeds at individual plant scale level. It was found that the close to crop area is like a ring structure, comprising an area between an inner- and outer-circle around the sugar beet seedling. Physical weeding should not be applied to the area within the inner circle. The radius of the inner circle increases with the appearance of young beet leaves during the growth season. It was also found, that no weeds were germinating within 1 cm around individual sugar beet seedlings. Therefore this distance should be added to the radius of the inner circle. The space between the inner and outer circle is termed the close to crop area where physical weeding should be applied. The size of this area is defined by the developmental stage of the sugar beet fibrous root system and foliage. Thus, the determination of the growth stage of individual crop plants is necessary before any physical weeding can take place in the close to crop area. Uprooting, cutting between stem and root or damage of main shoot can do the physical control of most weed species located in the close to crop area. However, the targeting of weeds from above and from different angels above ground is limited in the close to crop area. This is caused by the fact that sugar beet leaves do not leave much space between leaves and ground and that our own study indicate that 26.4% of sugar beet plants at the 4-6 leaf stage are covering the main shoot of weeds. The most problematic weeds are the species, which have their main shoot and leaves located close to ground level. These species can either be controlled by damage of the main shoot or with a combination of shallow surface cutting and burial. Discrimination between weed species is beneficial under certain circumstances. First, the efficiency of the physical control of individual weed species is depending on the timing. Secondly some weeds species do not have significant negative impact on the yield, but instead leaving these species uncontrolled could benefit to an increased bio-diversity and reduced time and energy input for a physical weeding process. This paper is contributing to the ongoing Danish research project Robotic Weeding

Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum)

To develop an efficient protocol for shoot regeneration of root chicory (Cichorium intybus var. sativum), some factors, including different concentrations of plant growth regulators in Murashige and Skoog (MS) medium, type of explants and genotypes were evaluated. Initiation of callusing were best achieved in MS medium supplemented with 1-naphthaleneacetic acid (NAA) (0.1 mg l-1) plus 6-Benzylaminopurine (6-BAP) (1 mg l-1), indole-3-acetic acid (IAA) (0.01 mg l-1) plus 6-BAP (1.0 mg l-1), and IAA (0.5 mg l-1) plus (0.5 mg l-1) 6-BAP combinations on leaf and cotyledon explants. Explant-derived calli were able to produce multiple adventitious shoots in MS medium containing IAA (0.5 mg l-1) plus 6-BAP (0.5 mg l-1). MS medium containing indole-3-butylric acid IBA (1 mgl-1) efficiently induced rooting on elongated shoots. Various responses to the number of generated shoots were observed when regeneration abilities of different chicory cultivars were examined. Among root and “Witloof” cultivars, ‘Melci’ and ‘Hera’ belong to the root cultivars and exhibited higher shoot regeneration ability. Using the optimized regeneration method, genetic transformation of ‘Melci’ with Agrobacterium tumefaciens strain C58C1 RifR (pGV2260) (pTJK136) was successfully carried out. Histochemical GUS assay, polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis of putative transformed plants confirmed successful integration of the T-DNA into the chicory genome. Expression of the neomycine phosphotransferase (NPTII) in the regenerated plants was also shown by well-developed roots on root inducing medium containing 100 mg l-1 kanamycin. This simple, efficient and reproducible protocol could be useful for inducing somaclonal variation and genetic modification of root chicory cultivars to broaden genetic variation and transferring of important genes

Differential Effects of Increasing Salinity on Germination and Seedling Growth of Native and Exotic Invasive Cordgrasses

Soil salinity is a key environmental factor influencing germination and seedling establishment in salt marshes. Global warming and sea level rise are changing estuarine salinity, and may modify the colonization ability of halophytes. We evaluated the effects of increasing salinity on germination and seedling growth of native Spartina maritima and invasive S. densiflora from wetlands of the Odiel-Tinto Estuary. Responses were assessed following salinity exposure from fresh water to hypersaline conditions and germination recovery of non-germinated seeds when transferred to fresh water. The germination of both species was inhibited and delayed at high salinities, while pre-exposure to salinity accelerated the speed of germination in recovery assays compared to non-pre-exposed seeds. S. densiflora was more tolerant of salinity at germination than S. maritima. S. densiflora was able to germinate at hypersalinity and its germination percentage decreased at higher salinities compared to S. maritima. In contrast, S. maritima showed higher salinity tolerance in relation to seedling growth. Contrasting results were observed with differences in the tidal elevation of populations. Our results suggest S. maritima is a specialist species with respect to salinity, while S. densiflora is a generalist capable of germination of growth under suboptimal conditions. Invasive S. densiflora has greater capacity than native S. maritima to establish from seed with continued climate change and sea level rise.Ministerio de Educación, Cultura y Deporte (FPU14/06556

An Inquiry-Infused Introductory Biology Laboratory That Integrates Mendel\u27s Pea Phenotypes With Molecular Mechanisms

We developed a multi-week laboratory in which college-level introductory biology students investigate Mendel\u27s stem length phenotype in peas. Students collect, analyze and interpret convergent evidence from molecular and physiological techniques. In weeks 1 and 2, students treat control and experimental plants with Gibberellic Acid (GA) to determine whether uncharacterized short mutant lines are GA responsive. These data allow students to place the mutation in the GA signal transduction pathway. During weeks 2 and 3, plants are genotyped for Mendel\u27s le mutation using a derived cleaved polymorphic sequences (dCAPS) PCR assay. This laboratory allows students to make a direct connection between modern molecular genetics and the easily scored phenotypes Mendel used as the basis of his fundamental discoveries. We administered surveys to assess student gains in accord with four learning goals: understanding the lab, basic science literacy, scientific practices, and working collaboratively. Student confidence increased significantly in the first three, but not in working collaboratively, although students reported greater confidence working in groups than alone

Mutations in UDP-Glucose:Sterol Glucosyltransferase in Arabidopsis Cause Transparent Testa Phenotype and Suberization Defect in Seeds

In higher plants, the most abundant sterol derivatives are steryl glycosides (SGs) and acyl SGs. Arabidopsis (Arabidopsis thaliana) contains two genes, UGT80A2 and UGT80B1, that encode UDP-Glc:sterol glycosyltransferases, enzymes that catalyze the synthesis of SGs. Lines having mutations in UGT80A2, UGT80B1, or both UGT80A2 and UGT8B1 were identified and characterized. The ugt80A2 lines were viable and exhibited relatively minor effects on plant growth. Conversely, ugt80B1 mutants displayed an array of phenotypes that were pronounced in the embryo and seed. Most notable was the finding that ugt80B1 was allelic to transparent testa15 and displayed a transparent testa phenotype and a reduction in seed size. In addition to the role of UGT80B1 in the deposition of flavanoids, a loss of suberization of the seed was apparent in ugt80B1 by the lack of autofluorescence at the hilum region. Moreover, in ugt80B1, scanning and transmission electron microscopy reveals that the outer integument of the seed coat lost the electron-dense cuticle layer at its surface and displayed altered cell morphology. Gas chromatography coupled with mass spectrometry of lipid polyester monomers confirmed a drastic decrease in aliphatic suberin and cutin-like polymers that was associated with an inability to limit tetrazolium salt uptake. The findings suggest a membrane function for SGs and acyl SGs in trafficking of lipid polyester precursors. An ancillary observation was that cellulose biosynthesis was unaffected in the double mutant, inconsistent with a predicted role for SGs in priming cellulose synthesis

Seasonal Abundance; Damage; and Comparison of Different Heights, Orientations, and Directions of Yellow Sticky Traps for Sampling of \u3ci\u3eLiriomyza Trifolii\u3c/i\u3e (Diptera: Agromyzidae) in Cotton

This study was done to determine the optimum height, orientation, and direction of yellow sticky traps for sampling of Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) in cotton fields. Leaf infestation rates for the whole plants, as well as for each of three plant regions, and number of mines per infested leaf were also determined. Traps were placed at 25 cm above the plant canopy and 30 cm above the ground at two orientations (horizontal and vertical) and two directions (east-to-west and north-to-south) for each height and orientation in Aydin province, Turkey. Horizontally oriented traps captured more flies than vertically oriented traps. More flies were captured 25 cm above the plant canopy than 30 cm above the ground. The upper sides of horizontally oriented traps had the highest capture rates. Direction of traps did not significantly affect capture rate. The infestation rate of leaves was highest (14.1%) the first week of August and generally higher lower on the plant (79.4-100%). No mines were found in the upper region (top third) of the plants. No adults emerged from reared mines. These results are useful in development of sampling protocols to aid growers in making decisions and could be used as an additional component in Integrated Pest Management against L. trifolii in cotton fields

Genetic, evolutionary and plant breeding insights from the domestication of maize.

The natural history of maize began nine thousand years ago when Mexican farmers started to collect the seeds of the wild grass, teosinte. Invaluable as a food source, maize permeated Mexican culture and religion. Its domestication eventually led to its adoption as a model organism, aided in large part by its large chromosomes, ease of pollination and growing agricultural importance. Genome comparisons between varieties of maize, teosinte and other grasses are beginning to identify the genes responsible for the domestication of modern maize and are also providing ideas for the breeding of more hardy varieties

A classification of soybeans

Publication authorized November 7, 1929.Includes index